DIETS THAT HAVE POTENTIAL TO STIMULATE THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS IN SPRAGUE DAWLEY RATS

The pituitary gland is the master which regulates body hormones. From corticotrophs of anterior gland, adrenocorticotrophic hormone (ACTH) secreted and influences corticosterone production in rodents via hypothalamic–pituitary–adrenal axis (HPA axis). ACTH are frequently used as a hormonal marker stress level. Disturbance were observed many diseases especially Addison’s disease, Cushing’s syndrome, metabolic syndrome. Our objective to observe diet produces most least Sprague Dawley rat’s physiology. Method: Thirty-five rats grouped into five groups (n=7) given different types diets (control, high-fat, high-protein, high-sugar, high-starch) with tap water supplied drinking ad libitum. By end eight weeks, blood was collected, serum separated. individual components from extracted, purified, identified, quantified using High-Performance Liquid Chromatography (HPLC) photodiode array (PDA) analysis method. Result: analytical studies revealed high -sugar registered highest It also that high-fat high-sugar group presented peak for corticosterone. Conclusion: Consumption weeks suggested induce physiologic evidenced by HPA activation.